Cell Based Assays
Discovery Life Sciences Gentest Sprague DawlRat Hep
Gentest Sprague Dawley Rat Hepatocytes Cryopreserved Female Plateable Inducible
Eurofins Discoverx PathHunter eXpress GPR109B CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0791E2CP2M)
The PathHunter GPR109B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 3-hydroxyoctanoic acid) based activation of GPR109B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR109B and EA-tagged β-Arrestin-2. Activation of GPR109B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Phalloidin-iFluor 405 Reagent
Phalloidin-iFluor 405 Reagent
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Heme Assay Kit sufficient
Heme is a member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm and is a key prosthetic group for various essential proteins such as hemoglobin cytochromes catalases and peroxidases. free heme which can be released from hemoglobin following hemolysis is pro-inflammatory and contributes to iron-derived reactive oxygen species. Heme determination is widely practiced by researchers of various blood diseases.
AKADEUM LIFE SCIENCES INC Akadeum DEAD CELL REMOVAL Microbubble Kit
The Dead Cell Removal Microbubble Kit is designed to deplete apoptotic cells from a variety of biological samples. During apoptosis, phosphatidylserine (PS) – a phospholipid normally found on the inner surface of the plasma membrane – is redistributed to the extracellular surface. The relocation of PS to the outer surface of the cell membrane provides an effective target for differentiating dead cells from viable cells within a biological sample. The Dead Cell Removal Kit utilizes BACS™ Microbubbles coated with Annexin V, a PS binding molecule, to capture and float dead cells away. How it Works: The Dead Cell Removal Microbubble Kit are mixed into the sample where they bind to dead and dying cells. Following a centrifugation step, the microbubbles and dead cells are aspirated away while enriched cells are left healthy, untouched, and ready for a wide variety of applications such as single-cell techniques, flow sorting, and cell culturing. Capacity: 1mL
Eurofins Discoverx PathHunter eXpress EphB3 Functional Assay, 10-Plate Kit. (93-0842E3CP16L)
The PathHunter eXpress EphB3 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB3 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphB3 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical FFAR1GPR40 Reporter asy 1ea
Coriolis consumables ensure optimal collection efficiency for the Coriolis Air Sampler.
Eurofins Discoverx PathHunter eXpress PTGER3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0640E2CP0L)
The PathHunter PTGER3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER3 and EA-tagged β-Arrestin-2. Activation of PTGER3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
BioVendor, LLC Sircol; INSOLUBLE Collagen Assay, Bovine Collagen (Large Economy Kit Size: 400 assays), Multispecies colorimetric assay, Biocolor Item # S6000
Sircol; INSOLUBLE Collagen Assay, Bovine Collagen (Large Economy Kit Size: 400 assays). The Sircol; INSOLUBLE Collagen Assay is a dye-binding method for analysis of insoluble collagen fibers. The assay be used to assess the rate of production of newly laid down collagen fibers during periods of rapid growth, development, tissue repair, remodeling and wound healing. Sources of material includes tissues, bone and calcified tissue. It replaces the conventional hydroxyproline analysis. No more working with concentrated acids or alkalis! The Sircol Insoluble Assay uses mild acid and temperature treatment for 2 to 3 hrs. The solubility conversion to denatured collagen and subsequent measurement can be completed within one working day. The ratio of soluble and insoluble collagens can be measured using the same Sircol Dye Reagent. Multispecies colorimetric assay. Detection Limit: 10.0 g. Assay-time: 4 hours. Biocolor Item # S6000
Abcam SIRT2 Activity Assay Kit (Fluorometric)
SIRT2 Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical Glycogen phsphoylase InHBI 1mg
A cell-permeable acyl urea first identified as an inhibitor of human liver glycogen phosphorylase (IC50 = 53 nM); blocks glucagon-induced hepatic glycogenolysis in vivo
Eurofins Discoverx PathHunter eXpress TYK2 Activity Assay, 2-Plate Kit. (93-0875E3CP16M)
The PathHunter eXpress TYK2 Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the TYK kinase domain. The cell line is engineered to express the catalytic domain from TYK2 fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the TYK2 catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL17RA/IL17RC Dimerization Bioassay Kit, 2pl. (93-0999Y3-00053)
The PathHunter IL17RA/IL17RC Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-17) induced dimerization of IL17RA/IL17RC receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Abcam Thrombin Activity Assay Kit (Fluorometric)
Thrombin Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical CellBased Assay Buffer Tablet
Cell-Based Assay Buffer Tablet has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.